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phosphorylated stat1 9167s antibody  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated stat1 9167s antibody
    Phosphorylated Stat1 9167s Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated stat1 9167s antibody/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    phosphorylated stat1 9167s antibody - by Bioz Stars, 2026-02
    90/100 stars

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    phosphorylated stat1 9167s antibody  (Cell Signaling Technology Inc)
    90
    Cell Signaling Technology Inc phosphorylated stat1 9167s antibody
    Phosphorylated Stat1 9167s Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/phosphorylated stat1 9167s antibody/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
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    phosphorylated tyr701 stat1 9167  (Cell Signaling Technology Inc)
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    Fig. 5 The AIM2-triggered IL-1β secretion positively regulates <t>STAT1/NF-κB</t> activation, irradiation resistance, cellular migration ability and PD-L1 expression in OSCC cells. A Dot blot (upper) of three independent experiments (Exp) and histograms (lower) of ELISA results for the IL-1β protein levels in the culture media from HSC4 (left) and SAS (right) cell variants. B Western blot analyses for phosphorylated STAT1/NF-κB, total STAT1/NF-κB and GAPDH protein levels (upper) and histograms for DNA-binding activity of STAT1/NF-κB in luciferase reporter assays (lower) against the AIM2-overexpressing SAS cells treated without or with IL-1β-neutralizing or isotype antibody (Ab, 10 μg/ml of each). C–H Crystal violet staining (upper)/histogram (lower) for colony formation post-treatment without or with irradiation at 4 Gy, Giemsa staining (upper)/ histogram (lower) for the migrated cells and RT-PCR/Western blot analyses for the mRNA/protein levels of PD-L1 and GAPDH (upper)/histograms for PD-L1 transcriptional activity in luciferase assays (lower) against the AIM2-overexpressing SAS cells cultivated in the absence or presence of IL-1β-neutralizing/isotype antibody (C–E) and NF-κB inhibitor SN50 at the indicated concentrations (F–H). In B, E and H, GAPDH was used as an internal control of experiments. The symbol “***” and different alphabets represent p < 0.001 and p < 0.05, respectively
    Phosphorylated Tyr701 Stat1 9167, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    phosphorylated stat1 antibody  (Cell Signaling Technology Inc)
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    Fig. 5 The AIM2-triggered IL-1β secretion positively regulates <t>STAT1/NF-κB</t> activation, irradiation resistance, cellular migration ability and PD-L1 expression in OSCC cells. A Dot blot (upper) of three independent experiments (Exp) and histograms (lower) of ELISA results for the IL-1β protein levels in the culture media from HSC4 (left) and SAS (right) cell variants. B Western blot analyses for phosphorylated STAT1/NF-κB, total STAT1/NF-κB and GAPDH protein levels (upper) and histograms for DNA-binding activity of STAT1/NF-κB in luciferase reporter assays (lower) against the AIM2-overexpressing SAS cells treated without or with IL-1β-neutralizing or isotype antibody (Ab, 10 μg/ml of each). C–H Crystal violet staining (upper)/histogram (lower) for colony formation post-treatment without or with irradiation at 4 Gy, Giemsa staining (upper)/ histogram (lower) for the migrated cells and RT-PCR/Western blot analyses for the mRNA/protein levels of PD-L1 and GAPDH (upper)/histograms for PD-L1 transcriptional activity in luciferase assays (lower) against the AIM2-overexpressing SAS cells cultivated in the absence or presence of IL-1β-neutralizing/isotype antibody (C–E) and NF-κB inhibitor SN50 at the indicated concentrations (F–H). In B, E and H, GAPDH was used as an internal control of experiments. The symbol “***” and different alphabets represent p < 0.001 and p < 0.05, respectively
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    Fig. 5 The AIM2-triggered IL-1β secretion positively regulates <t>STAT1/NF-κB</t> activation, irradiation resistance, cellular migration ability and PD-L1 expression in OSCC cells. A Dot blot (upper) of three independent experiments (Exp) and histograms (lower) of ELISA results for the IL-1β protein levels in the culture media from HSC4 (left) and SAS (right) cell variants. B Western blot analyses for phosphorylated STAT1/NF-κB, total STAT1/NF-κB and GAPDH protein levels (upper) and histograms for DNA-binding activity of STAT1/NF-κB in luciferase reporter assays (lower) against the AIM2-overexpressing SAS cells treated without or with IL-1β-neutralizing or isotype antibody (Ab, 10 μg/ml of each). C–H Crystal violet staining (upper)/histogram (lower) for colony formation post-treatment without or with irradiation at 4 Gy, Giemsa staining (upper)/ histogram (lower) for the migrated cells and RT-PCR/Western blot analyses for the mRNA/protein levels of PD-L1 and GAPDH (upper)/histograms for PD-L1 transcriptional activity in luciferase assays (lower) against the AIM2-overexpressing SAS cells cultivated in the absence or presence of IL-1β-neutralizing/isotype antibody (C–E) and NF-κB inhibitor SN50 at the indicated concentrations (F–H). In B, E and H, GAPDH was used as an internal control of experiments. The symbol “***” and different alphabets represent p < 0.001 and p < 0.05, respectively
    Antibodies Against Tyrosine Phosphorylated Stats, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti phosphorylated stat1 antibody  (Cell Signaling Technology Inc)
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    Fig. 5 The AIM2-triggered IL-1β secretion positively regulates <t>STAT1/NF-κB</t> activation, irradiation resistance, cellular migration ability and PD-L1 expression in OSCC cells. A Dot blot (upper) of three independent experiments (Exp) and histograms (lower) of ELISA results for the IL-1β protein levels in the culture media from HSC4 (left) and SAS (right) cell variants. B Western blot analyses for phosphorylated STAT1/NF-κB, total STAT1/NF-κB and GAPDH protein levels (upper) and histograms for DNA-binding activity of STAT1/NF-κB in luciferase reporter assays (lower) against the AIM2-overexpressing SAS cells treated without or with IL-1β-neutralizing or isotype antibody (Ab, 10 μg/ml of each). C–H Crystal violet staining (upper)/histogram (lower) for colony formation post-treatment without or with irradiation at 4 Gy, Giemsa staining (upper)/ histogram (lower) for the migrated cells and RT-PCR/Western blot analyses for the mRNA/protein levels of PD-L1 and GAPDH (upper)/histograms for PD-L1 transcriptional activity in luciferase assays (lower) against the AIM2-overexpressing SAS cells cultivated in the absence or presence of IL-1β-neutralizing/isotype antibody (C–E) and NF-κB inhibitor SN50 at the indicated concentrations (F–H). In B, E and H, GAPDH was used as an internal control of experiments. The symbol “***” and different alphabets represent p < 0.001 and p < 0.05, respectively
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    rabbit anti 167 phosphorylated stat1 antibody  (Cell Signaling Technology Inc)
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    Fig. 5 The AIM2-triggered IL-1β secretion positively regulates <t>STAT1/NF-κB</t> activation, irradiation resistance, cellular migration ability and PD-L1 expression in OSCC cells. A Dot blot (upper) of three independent experiments (Exp) and histograms (lower) of ELISA results for the IL-1β protein levels in the culture media from HSC4 (left) and SAS (right) cell variants. B Western blot analyses for phosphorylated STAT1/NF-κB, total STAT1/NF-κB and GAPDH protein levels (upper) and histograms for DNA-binding activity of STAT1/NF-κB in luciferase reporter assays (lower) against the AIM2-overexpressing SAS cells treated without or with IL-1β-neutralizing or isotype antibody (Ab, 10 μg/ml of each). C–H Crystal violet staining (upper)/histogram (lower) for colony formation post-treatment without or with irradiation at 4 Gy, Giemsa staining (upper)/ histogram (lower) for the migrated cells and RT-PCR/Western blot analyses for the mRNA/protein levels of PD-L1 and GAPDH (upper)/histograms for PD-L1 transcriptional activity in luciferase assays (lower) against the AIM2-overexpressing SAS cells cultivated in the absence or presence of IL-1β-neutralizing/isotype antibody (C–E) and NF-κB inhibitor SN50 at the indicated concentrations (F–H). In B, E and H, GAPDH was used as an internal control of experiments. The symbol “***” and different alphabets represent p < 0.001 and p < 0.05, respectively
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    Fig. 5 The AIM2-triggered IL-1β secretion positively regulates STAT1/NF-κB activation, irradiation resistance, cellular migration ability and PD-L1 expression in OSCC cells. A Dot blot (upper) of three independent experiments (Exp) and histograms (lower) of ELISA results for the IL-1β protein levels in the culture media from HSC4 (left) and SAS (right) cell variants. B Western blot analyses for phosphorylated STAT1/NF-κB, total STAT1/NF-κB and GAPDH protein levels (upper) and histograms for DNA-binding activity of STAT1/NF-κB in luciferase reporter assays (lower) against the AIM2-overexpressing SAS cells treated without or with IL-1β-neutralizing or isotype antibody (Ab, 10 μg/ml of each). C–H Crystal violet staining (upper)/histogram (lower) for colony formation post-treatment without or with irradiation at 4 Gy, Giemsa staining (upper)/ histogram (lower) for the migrated cells and RT-PCR/Western blot analyses for the mRNA/protein levels of PD-L1 and GAPDH (upper)/histograms for PD-L1 transcriptional activity in luciferase assays (lower) against the AIM2-overexpressing SAS cells cultivated in the absence or presence of IL-1β-neutralizing/isotype antibody (C–E) and NF-κB inhibitor SN50 at the indicated concentrations (F–H). In B, E and H, GAPDH was used as an internal control of experiments. The symbol “***” and different alphabets represent p < 0.001 and p < 0.05, respectively

    Journal: Journal of translational medicine

    Article Title: AIM2 promotes irradiation resistance, migration ability and PD-L1 expression through STAT1/NF-κB activation in oral squamous cell carcinoma.

    doi: 10.1186/s12967-023-04825-w

    Figure Lengend Snippet: Fig. 5 The AIM2-triggered IL-1β secretion positively regulates STAT1/NF-κB activation, irradiation resistance, cellular migration ability and PD-L1 expression in OSCC cells. A Dot blot (upper) of three independent experiments (Exp) and histograms (lower) of ELISA results for the IL-1β protein levels in the culture media from HSC4 (left) and SAS (right) cell variants. B Western blot analyses for phosphorylated STAT1/NF-κB, total STAT1/NF-κB and GAPDH protein levels (upper) and histograms for DNA-binding activity of STAT1/NF-κB in luciferase reporter assays (lower) against the AIM2-overexpressing SAS cells treated without or with IL-1β-neutralizing or isotype antibody (Ab, 10 μg/ml of each). C–H Crystal violet staining (upper)/histogram (lower) for colony formation post-treatment without or with irradiation at 4 Gy, Giemsa staining (upper)/ histogram (lower) for the migrated cells and RT-PCR/Western blot analyses for the mRNA/protein levels of PD-L1 and GAPDH (upper)/histograms for PD-L1 transcriptional activity in luciferase assays (lower) against the AIM2-overexpressing SAS cells cultivated in the absence or presence of IL-1β-neutralizing/isotype antibody (C–E) and NF-κB inhibitor SN50 at the indicated concentrations (F–H). In B, E and H, GAPDH was used as an internal control of experiments. The symbol “***” and different alphabets represent p < 0.001 and p < 0.05, respectively

    Article Snippet: After blocking step, the membranes were further incubated with primary antibodies against PD-L1 (GTX635975, GeneTex, Hsinchu, Taiwan), phosphorylated (Ser536) NF-κB (#3033)/NF-κB (#6956)/phosphorylated (Tyr701) STAT1 (#9167)/STAT1 (#9172) (Cell Signaling, Danvers, MA, USA) and GAPDH (#PA0212, AbFrontier, Seoul, Korea), IL-1β (#41,059, SAB) overnight at 4 °C.

    Techniques: Activation Assay, Irradiation, Migration, Expressing, Dot Blot, Enzyme-linked Immunosorbent Assay, Western Blot, Binding Assay, Activity Assay, Luciferase, Staining, Reverse Transcription Polymerase Chain Reaction, Control